Helpful tips

How do you calculate ET50?

How do you calculate ET50?

To determine the ET50-light for this trial, plot the percent leaf disk floating as a function of time: From the above graph, we can interpolate that 50% of the leaf disks floated at 5.5 minutes. Therefore, the ET50-light is 5.5 minutes.

Why would scientists typically Graph 1 ET50 instead of ET50?

Taking the reciprocal of ET50, 1/ ET50 allows the graphic presentation to more closely express the physical phenomenon, as shown in Figure 3. This procedure is particularly useful for comparing photosynthetic rates between C4 and C3 plants.

How does sodium bicarbonate affect photosynthesis?

From this investigation, we learned that an increase in carbon dioxide allows for photosynthesis to occur at a faster rate. The more sodium bicarbonate present, there is a greater amount of carbon dioxide present, which in result will increase the speed of photosynthesis.

How does cellophane affect photosynthesis?

Blue: If we place a chloroplast solution in a test tube wrapped in blue cellophane, then the chloroplasts will photosynthesize at small rate because the cellophane is reflecting blue light away from the chloroplasts and blue is a very crucial color to photosynthesize, so this deprivation of blue light would make the …

How is ET50 used to measure photosynthesis?

The ET50 is the time it takes for 50% of the leaf disks to float and is a good indicator of the rate of photosynthesis. It was hypothesized that if the concentration of carbon dioxide was increased, then the rate at which photosynthesis occurs will also increase.

What is the ratio of photosynthesis to respiration?

The magnitude of the error introduced by the respiratory loss of C14 is proportional to the ratio of respiration to photosynthesis. During exponential growth of Chlamydomonas, respiration is 5–10% of photosynthesis, but in nutrient starved, non-growing, cultures respiration may equal photosynthesis.

What color has the highest rate of photosynthesis?

red light
White light appears to be the most effective light for photosynthesis, but apart from white, red light is also effective. When white light is not used, red is more effective than its counterparts, most likely because red light has a long wavelength that allows it radiate more energy and heat than other colors.

Why did the leaf disks begin to float?

The biology behind the procedure: Leaf disks float, normally. When the air spaces are infiltrated with solution the overall density of the leaf disk increases and the disk sinks. As photosynthesis proceeds oxygen is released into the interior of the leaf, which changes the buoyancy–causing the disks to rise.

What is the purpose of baking soda in the photosynthesis lab?

The purpose of making this solution is that the bicarbonate serves as a source of carbon dioxide, which the leaf disks require, along with water and light energy, to undergo photosynthesis.

Why do you need to add carbon dioxide with the 3% sodium bicarbonate solution?

Why do you need to add carbon dioxide with the 3% sodium bicarbonate (NaHCO3) solution? Carbon dioxide can be used by plants to make glucose during photosynthesis.

What color has highest rate of photosynthesis?

White light is the most effective light for photosynthesis because it provides a wide range of colored lights for various pigments to use. When only a single color is used, red is the most effective color of light.

What is cellophane to light?

The cellophane absorbs other colours of light. For example, green light will not pass through red cellophane. The green light is absorbed by the red cellophane. However, green light will pass through green cellophane.

What is the process of photosynthesis in Elodea lab?

Photosynthesis in Elodea Lab Essay. Elodea & PhotosynthesisPhotosynthesis is the process by which green plants and some other organisms use sunlight to synthesize nutrients from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a by-product.

How to do ET50 value determination in photosynthesis?

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How to measure oxygen bubbles in Elodea plant?

Place the plant into a beaker.Place a source of light 5cm from plant.Wait a minuteAfter one minute, count and record the number of oxygen bubbles rising from the cut end of the stem for 3 minutes. If bubbles fail to appear after one minute, repeat part A.

What’s the best way to light up an Elodea plant?

Also, if the light is closer, the process will be faster. The procedure for this would be to leave the Elodea plant in water with sodium bicarbonate (baking soda increases carbon dioxide in water), set up the lamp at 5cm distance and start the timer for 3 minutes.