What is the purpose of the bacterial transformation lab?
Bacterial transformation is a key step in molecular cloning, the goal of which is to produce multiple copies of a recombinant DNA molecule. Prior steps for creating recombinant plasmids are described in traditional cloning basics and involve insertion of a DNA sequence of interest into a vector backbone.
What is the independent variable in the pGLO lab?
Independent variable: the addition of pGLO, arabinose, and ampicilin. Dependent variable: bacterial growth and if it glows. Constants: size of plates, temperature of water bath, temperature of ice bath, measurement unit, amount of pGLO/ampicilin, etc.
What is responsible for bacterial transformation?
DNA
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. It was first reported in Streptococcus pneumoniae by Griffith in 1928. DNA as the transforming principle was demonstrated by Avery et al in 1944.
What is the purpose of DNA transformation?
Transformation of cells is a widely used and versatile tool in genetic engineering and is of critical importance in the development of molecular biology. The purpose of this technique is to introduce a foreign plasmid into bacteria, the bacteria then amplifies the plasmid, making large quantities of it.
How do you know if transformation is successful?
How can you tell if a transformation experiment has been successful? If transformation is successful, the DNA will be integrated into one of the cell’s chromosomes. How are genetic markers related to transformation?
Where is pGLO from?
The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from the jelly fish Aequorea victoria.
How can you tell if a transformation experiment has been successful?
How can you tell if a transformation experiment has been successful? If transformation is successful, the DNA will be integrated into one of the cell’s chromosomes. You just studied 9 terms!
What happens during DNA transformation?
Plasmid or vector transformation is the process by which exogenous DNA is transferred into the host cell. Transformation usually implies uptake of DNA into bacterial, yeast or plant cells, while transfection is a term usually reserved for mammalian cells.
How can you tell if a transformation experiment is successful?
How can you tell if a transformation experiment has been successful? If transformation is successful, the DNA will be integrated into one of the cell’s chromosomes.