Is Mycobacterium smegmatis anaerobic?
Mycobacterium is a dominant genus in the soil, and all its species are obligate aerobes. Here we show that an obligate aerobe, the soil actinomycete Mycobacterium smegmatis, adopts an anaerobe-type strategy by activating fermentative hydrogen production to adapt to hypoxia.
Does Mycobacterium smegmatis grow on blood agar?
Mycobacterium smegmatis phenotypic characterization. A- Blood agar cultivation showing sparse growth of lightly pigmented, shiny and smooth colonies. B- Ziehl-Neelsen staining with poorly pigmented, irregular, slender acid-fast rods [magnification: 40x].
Where does Mycobacterium smegmatis grow?
Mycobacterium smegmatis are mostly found in the soil, water, and plants. They tend mostly to exist near large bodies of water. Isolates have been discovered in 16 States, Australia, Russia, Canada, and Switzerland (1).
Is Mycobacterium smegmatis cocci or bacilli?
Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinobacteria and the genus Mycobacterium. It is 3.0 to 5.0 µm long with a bacillus shape and can be stained by Ziehl–Neelsen method and the auramine-rhodamine fluorescent method.
Why was Mycobacterium smegmatis used instead?
An acid fast bacterium holds onto the dye. Why was Mycobacterium smegmatis used instead of Mycobacterium tuberculosis in this exercise? protect bacteria from bacteriophages & allow bacteria to survive longer in the host & cause disease.
What color does Staphylococcus aureus stain in acid fast?
It is red because it is acid fast positive. The carbolfuchsin is more soluble in the cell wall lipids therefore, retains the red color. What color is Bacillus megaterium in an acid fast stain? Why?
Can Mycobacterium be cured?
Can nontuberculous mycobacteria (NTM) disease be cured? A cure for NTM is possible and long-term success rates of treating this infection can be as high as 86%. If a cure is not possible, treatment may allow for stabilization of lung disease and prevention of continued lung destruction.
What makes Mycobacterium smegmatis a Gram positive bacteria?
Mycobacterium smegmatis is a Gram-positive bacteria, characterized by an inner cell membrane and a thick cell wall. The Gram-positive bacteria is further classified as one with a high GC content and therefore a low AT content. This quality is used as a crude measure of similarity of different species of bacteria.
How does iron deficiency affect growth of Mycobacterium smegmatis?
Figure 4. Winder and O’Hara (1961, 1962) reported morphological changes during iron-deficient growth of Mycobacterium smegmatis. They found that under conditions of iron deficiency the length of the bacteria was increased severalfold as compared with cells grown in iron-sufficient medium.
How big is the fatty acid synthase complex of Mycobacterium smegmatis?
The fatty acid synthase complex of Mycobacterium smegmatis represents an exception to the general rule that the enzymes of bacterial fatty acid synthesis do not form multifunctional complexes. It was discovered that this bacterium has a fatty acid synthase complex composed of six identical subunits, each having a molecular mass of 290 kDa.
Is there a case of Mycobacterium smegmatis killing a human?
However, there has been 1 case where the bacteria has killed a human after infection. In this case, an Italian child was infected by Mycobacterium smegmatis and died at the age of 8. After genetic analysis of the child, it was determined that four nucleotides were inserted, causing a frameshift and a nonsense mutation.